Multilevel dynamics in rabbit embryonic development from in vivo multiscale imaging – Comparative approach with zebrafish for appendages patterning
Supervisor: Dr. N Peyriéras, co-supervisors: local: Dr Monique Frain, external: Dr P.Loza (P6)
- Characterise the processes underlying rabbit embryonic development throughout pre-implantation and implantation stages (ex vivo) from the in vivo observation of cell behaviours and the quantification of metabolic and signalling activities along the cell lineage;
- Further document the current hypothesis that rabbit development is closer to human than mouse development is at blastulation and gastrulation stages as well as at later stages for limb formation.
- Culture and image rabbit embryos through implantation stages (fluorescent staining; transgenic lines and/or new vital dyes);
- Analyse cohorts of individuals in defined genetic and environmental conditions to derive probabilistic models for the evolution of specific cellular features to construct prototypic 4D models of rabbit early embryogenesis;
- Challenge mechanical cues by manipulating the size of the inner cell population through ablations and transplantation ;
- Develop a complete methodology for the live investigation of appendage development using the zebrafish model and the formation of pectoral fins.
Secondments: UCAM, Dr M. Zernicka-Goetz; ICFO, Dr. Loza; Manchester Met, Dr. Doursat
Links to other projects: ESR 14 : for live imaging on SPIM and ESR 11 (modeling of embryogenesis).