Cell dynamics and genetic regulation in the zebrafish hindbrain morphogenesis
Supervisor: Dr Monique Frain, co-supervisors: local: Dr. Nadine Peyriéras, external: Dr. Pablo Loza (P6)
Objectives: Build a mechanogenetic model to characterise the processes underlying the segmentation of the hindbrain from the in vivo observation of cell behaviours and optomanipulation of signalling activities.
- Decipher the underlying genetic, molecular, cellular and biomechanical
- Investigate tissue biomechanics.
- Assess the multilevel consequences of RA signalling activation
- Monitor the role of FGF8 in fgf8 mutants through the in vivo 3D+time imaging and automated tracking of rescued cells (optogenetic activation of FGF8 expression).
- Challenge a mechanogenetic model designed in WP4 in collaboration with P8 (MANCHESTER MET).
- Characterise the cell behaviours shaping the zebrafish hindbrain and the cellular individual and collective response to genetic perturbations.
- Validate our predictive understanding of the hindbrain morphogenesis in normal and pathological conditions with a mechanogenetic model (WP4) integrating cell motility, proliferation, adhesion, signalling and gene expression dynamics.
- academic: P1 UM Dr G Lutfalla, Screening new mutants and transgenic lines P8 Manchester Met R Doursat, Designing a mechanogenetic model, parameter space exploration
- industrial: P11, PhaseView, Dr I Lyuboshenko, Imaging solution and optogenetic manipulation. P14 Union Biometrica, Dr D Strack, Microfluidics.
Links to other projects: Collaboration with ESR1 (reporters), ESR6 (developmental processes) ESR10 for microscopes, ESR12 for Modelling and with ESRs 14 for incubation chambers.